two-color microarray-based gene expression analysis microarrays  (Agilent technologies)

Journal: Frontiers in Oncology

Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro

doi: 10.3389/fonc.2024.1323422

Figure Lengend Snippet: Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by microarray. There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.

Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

Techniques: Microarray, Activation Assay

Journal: BMC Research Notes

Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish

doi: 10.1186/1756-0500-3-73

Figure Lengend Snippet: Validation of the RNA isolation method from single zebrafish embryos . (a) RNA RIN values and (b) yields from: Single, individual embryos (4); Semi-single, homogenized, pooled, and split embryo material (4, see text); Developmental, embryos from the 16-cell to 8-somite stage (8); Unfertilized, unfertilized eggs (30); Epibolic, embryos from dome stage to 90% epiboly (186). Stages and RNA yield could not be linked. Note that RIN values show overlap because of a single decimal place measurement accuracy. (c) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bar, foreground signal and scatter board bar, background signal). (d) Principal component analysis (PCA) of the unnormalized log 2 ratios (test/reference) from the Single and Semi-single samples. (e) Spearman correlations showing the similarity of the unnormalized microarray data from Single and Semi-single samples.

Article Snippet: Each hybridization mixture was made up from 300 ng 'Test' and 300 ng 'Reference' sample according to the Two-Color Microarray-Based Gene Expression Analysis Manual version 5.5 (Agilent Technologies).

Techniques: Isolation, Microarray

Journal: BMC Research Notes

Article Title: RNA isolation method for single embryo transcriptome analysis in zebrafish

doi: 10.1186/1756-0500-3-73

Figure Lengend Snippet: Dissecting zebrafish development with microarray analysis . (a) Eight selected embryos ranging from the 16-cell to 8-somite stage. (b) Mean, unnormalized log 2 signal intensities from microarray analysis (smooth bars, foreground signal and scatter board bars, background signal). (c) Principal component analysis (PCA) on unnormalized log 2 ratio data (test/reference) showing a 'developmental' curve starting at the 16-cell stage and ending at the 8-somite stage. (d) Spearman correlations between the samples reflect the developmental distance.

Article Snippet: Each hybridization mixture was made up from 300 ng 'Test' and 300 ng 'Reference' sample according to the Two-Color Microarray-Based Gene Expression Analysis Manual version 5.5 (Agilent Technologies).

Techniques: Microarray